Prevention of trypsin-induced shock in rats by the pancreatic secretory trypsin inhibitor

Abstract

Jönsson P, Ljungcrantz I, Ohlsson K. Prevention of trypsin-induced shock in rats by the pancreatic secretory trypsin inhibitor. Scand J Clin Lab Invest 1994; 54: 595-9.

Intravenously infused bovine trypsin, 75mgkg^−l during 3h, induced shock in rats which proved lethal. After 5 h, all the rats had died. In another group of rats receiving trypsin, the pancreatic secretory trypsin inhibitor, 75 mg kg⁻¹, was infused during 5 h. These rats all survived. After about 1 h, α₁-macroglobulin was found to be saturated in both groups, while kininogen cleavage, coinciding with a decline in arterial blood pressure, only occurred in the untreated group. Trypsin complex formation with α₁ inhibitor 3 and α₁-proteinase inhibitor was more pronounced in untreated rats. It is concluded that the pancreatic secretory trypsin inhibitor may function as an effective trypsin inhibitor in plasma.

Key Words:

• α₁-inhibitor 3
• α₁ macroglobulin
• α₁-proteinase inhibitor
• pancreatic secretory trypsin inhibitor
• trypsin