The protein concentration of blood coagulation factor VII can be measured equally well in plasma and serum

Abstract

In the Northwick Park Heart Study, the coagulant activity of factor VII (FVII: C) has been identified as a risk marker of ischaemic heart disease. In the fasting state, the protein concentration of FVII (FVII: Ag) might be an even better risk marker, because of the low coefficient of variation of the antigen assay. Today, most analyses are performed in plasma samples, as it is unknown whether FVII, to some extent, is consumed during coagulation. In the present study, we have investigated, whether FVII: Ag can be measured equally well in plasma and serum. FVII: Ag was measured in 88 plasma and serum samples. Results were compared by means of linear regression, where y = 0.984 x + 0.770, r = 0.96. No systematic variation existed between FVII: Ag in plasma and serum. The mean difference in FVII: Ag between plasma and serum was - 1.17 (SD 11.92) arbitrary units, compared with a mean difference of 0.18 (SD 8.31) arbitrary units between duplicate measurements of the same plasma dilution. Our findings indicate that there is a good agreement between FVII: Ag in plasma and serum.

Key Words:

• coagulation
• ELISA
• factor VII
• population studies
• serum