Release of cytokines and proteases from human peripheral blood mononuclear and polymorphonuclear cells following phagocytosis and LPS stimulation

Abstract

Release and cellular contents of pro- and anti-inflammatory cytokines, neutro-philic elastase and secretory leukocyte proteinase inhibitor (SLPI) were measured with enzyme-linked immunosorbent assay in peripheral blood mono- and polymorphonuclear cells stimulated with preopsonized yeast cells or lipopolysaccha-ride. Tumour necrosis factor alpha (TNFu) was also measured with a bioassay. TNFα production and soluble TNFα receptor I (sTNF RI) were demonstrated in the environment of both cell populations. The bioassay indicated levels of TNFα far below those detected by ELISA. The overall secretion of cytokines and their inhibitors was found to favour an anti-inflammatory balance in the environment of the stimulated cells. The interleukin-1 receptor antagonist (ILl-ra), compared with interleukin-1β (IL-1β), dominated the secretions from both cell types with a 100- to 1000-fold excess respectively. Most of the translated IL-lβ was not secreted but found associated with the cellular compartments. In contrast to lipopolysaccharide (LPS) stimulation, preopsonized yeast cells stimulated a massive release of elastase from neutrophil cells.

Key Words:

• cytokines
• interleukin-1
• interleukin-1 receptor antagonist
• leukocyte proteases
• secretory leukocyte proteinase inhibitor
• soluble tumour necrosis factor receptor I
• tumour necrosis factor