Evaluation of Hernocue® Blood Glucose Analyzer for the instant diagnosis of hypoglycaemia in newborns

Abstract

The aim of the study was to evaluate a portable photometer, HemoCue® Blood Glucose Analyzer, in the instant diagnosis of hypoglycaemia in newborns. The HemoCue is a simple, easy-to-handle photometer; with an analysis time of less than 240 s. it utilizes a modified glucose dehydrogenase method in 5 μl whole blood. The HemoCue method was compared to a hexokinase method for deproteinized whole blood in a total of 118 samples from 5X newborns. The linear regression for these samples was Y = 1.19x-1.02 (range 0.7-7.2 mmol/L), r = 0.90. Ten samples were ≤2.0 mmol/L with both methods and 37 samples were ≤2.0 mmol/L with the HemoCue method. The average difference (D) for each :sample (n = 118) and the standard deviation (SD) for the difference were 0.45±0.46 mmol/L. Blood samples with a mean value with both methods ≤2.0 mmol/L (n = 20) had a D and SD of 0.71±0.29 mmol/L. When testing for linearity at low glucose concentrations, the HemoCue method gave significantly lower values compared to an ideal line. The HemoCue method has several advantages in the analysis of glucose in newborns: short analysis time, small sample size, and no influence from glycolysis. However. in our investigation, l'alsely low values occurred, especially in the low measuring range, so the HemoCue method is not suitable in the diagnosis of hypoglycaemia in newborns.

Key Words:

• Glucose dehydrogenase
• hexokinase