Experimental Pancreatitis in the Dog. Appearance of Complexes Between Proteases and Trypsin Inhibitors in Ascitic Fluid, Lymph, and Plasma

Abstract

Pancreatitis was initiated in dogs by creating a closed duodenal loop. Blood. lymph, and ascitic fluid were sampled throughout the disease. The changes in homogeneity of the plasma protease inhibitors were followed with immunochemical techniques. The following results were obtained. 1) BAPNA- and SUPHEPA-splitting (see p. 646) α-macroglobulin complexes were demonstrated early in the ascitic fluid and later in pancreatico-duodenal venous blood and lymph but not in the peripheral venous blood. Finally, all of the α-macroglobulins of the ascitic fluid were complexed. This was followed by rapid complexation of the α₁-antitrypsin of the ascitic fluid. 2) The trypsin-inhibiting capacity decreased by about 30% and 80% respectively in the peripheral venous blood and in the ascitic fluid in the course of pancreatitis. 3) When all the α-macroglobulins of the ascitic fluid were complexed, the blood pressure began to fall successively and the animals died.

Key-words:

• Ascitic fluid
• chymotrypsin
• lymph
• lymph nodes
• pancreatitis
• reticuloendothelial system
• trypsin