Incorporation of ³H-Thymidine into Deoxyribonucleic Acid of Mouse Small Intestine In Vitro

Abstract

³H-thymidine incorporation into DNA during in vitro culture of segments of the small intestine of the mouse was studied as a criterion of the viability of the crypt cells. Incubation of segments on Millipore filter in the interface between the organ culture medium (T 8) and the gaseous phase of 95% oxygen and 5% of carbon dioxide permitted the crypt cells of the intestine to proliferate rather normally after 180 minutes in culture.

Key-words:

• DNA, biosynthesis
• ileum
• intestine, small
• nucleotides, metabolism
• radiobiology
• thymidine.