8
Views
60
CrossRef citations to date
0
Altmetric
Original article

Coenzyme-A-Dependent Esterification of Cholesterol in Intestinal Mucosa from Guinea-Pig. Influence of Diet on the Enzyme Activity

, &
Pages 281-288 | Received 05 Oct 1976, Accepted 13 Nov 1976, Published online: 23 Feb 2010
 

Abstract

Guinea-pig intestinal mucosa contains a microsomal enzyme which catalyses the esterification of cholesterol. The enzyme is CoA-dependent and probably an acyl-CoA-cholesterol acyltransferase (ACAT) (E.C.2.3.1.26). The apparent Km for the CoA in the combined enzyme reaction is 3.3 X 10−5M. The specific activity of cholesterol esterification is about five times higher in young suckling animals than in ordinary fed adult animals. Feeding fat and fat/cholesterol diets increases the activity two- to five-fold. Concomitantly the fat/cholesterol fed animals get a marked accumulation of free and esterified cholesterol in mucosal cells.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.