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Abstract
Brush border membrane vesicles (BBMV) and basolateral membrane vesicles (BLMV) were simultaneously prepared from surgically resected pieces of morphologically intact human duodenum with a modified Percoll gradient centrifugation method. Alkaline phosphatase was enriched 20-fold in BBMV, whereas (Na+ + K+)-stimulated adenosine triphosphatase was enriched 15-fold in BLMV. BBMV and BLMV were preincubated with 3 pM synthetic somatostatin-14 or 3 μM SMS 201–995 for 10min at 5°C. In BBMV calcium, sodium, d-glucose, L-alanine, and D-mannitol uptake was unaffected by somatostatin-14 and SMS 201–995. In BLMV wc found two Ca++ transport systems: Na+/Ca++ exchange and ATP-driven Ca++ transport. Somatostatin-14 had no effect on either of the two transport mechanisms. SMS 201–995 had no effect on Na+/Ca++ exchange but significantly inhibited basolateral ATP-dependent Ca++ transport (−40% ± 5%. p < 0.005).