The Nasal Polyps as a Tool for Basic Research in Cystic Fibrosis

W. Bautsch Cystic Fibrosis Research Group, Dept. of Biophysical Chemistry and Dept. of Human Genetics, School of Medicine, Hannover; Physical Chemistry Institute, Ruprecht-Karls University, Heidelberg; Pediatric Clinic, Christian-Albrechts University, Kiel, FRG

N. Ponelies Cystic Fibrosis Research Group, Dept. of Biophysical Chemistry and Dept. of Human Genetics, School of Medicine, Hannover; Physical Chemistry Institute, Ruprecht-Karls University, Heidelberg; Pediatric Clinic, Christian-Albrechts University, Kiel, FRG

T. Darnedde Cystic Fibrosis Research Group, Dept. of Biophysical Chemistry and Dept. of Human Genetics, School of Medicine, Hannover; Physical Chemistry Institute, Ruprecht-Karls University, Heidelberg; Pediatric Clinic, Christian-Albrechts University, Kiel, FRG

K. Fryburg Cystic Fibrosis Research Group, Dept. of Biophysical Chemistry and Dept. of Human Genetics, School of Medicine, Hannover; Physical Chemistry Institute, Ruprecht-Karls University, Heidelberg; Pediatric Clinic, Christian-Albrechts University, Kiel, FRG

D. Grothues Cystic Fibrosis Research Group, Dept. of Biophysical Chemistry and Dept. of Human Genetics, School of Medicine, Hannover; Physical Chemistry Institute, Ruprecht-Karls University, Heidelberg; Pediatric Clinic, Christian-Albrechts University, Kiel, FRG

J. Hundrieser Cystic Fibrosis Research Group, Dept. of Biophysical Chemistry and Dept. of Human Genetics, School of Medicine, Hannover; Physical Chemistry Institute, Ruprecht-Karls University, Heidelberg; Pediatric Clinic, Christian-Albrechts University, Kiel, FRG

K. Miller Cystic Fibrosis Research Group, Dept. of Biophysical Chemistry and Dept. of Human Genetics, School of Medicine, Hannover; Physical Chemistry Institute, Ruprecht-Karls University, Heidelberg; Pediatric Clinic, Christian-Albrechts University, Kiel, FRG

S. Monajembashi Cystic Fibrosis Research Group, Dept. of Biophysical Chemistry and Dept. of Human Genetics, School of Medicine, Hannover; Physical Chemistry Institute, Ruprecht-Karls University, Heidelberg; Pediatric Clinic, Christian-Albrechts University, Kiel, FRG

A. Claab Cystic Fibrosis Research Group, Dept. of Biophysical Chemistry and Dept. of Human Genetics, School of Medicine, Hannover; Physical Chemistry Institute, Ruprecht-Karls University, Heidelberg; Pediatric Clinic, Christian-Albrechts University, Kiel, FRG

K. O. Greulich Cystic Fibrosis Research Group, Dept. of Biophysical Chemistry and Dept. of Human Genetics, School of Medicine, Hannover; Physical Chemistry Institute, Ruprecht-Karls University, Heidelberg; Pediatric Clinic, Christian-Albrechts University, Kiel, FRG

G. Maaβ Cystic Fibrosis Research Group, Dept. of Biophysical Chemistry and Dept. of Human Genetics, School of Medicine, Hannover; Physical Chemistry Institute, Ruprecht-Karls University, Heidelberg; Pediatric Clinic, Christian-Albrechts University, Kiel, FRG

B. Tömmler Cystic Fibrosis Research Group, Dept. of Biophysical Chemistry and Dept. of Human Genetics, School of Medicine, Hannover; Physical Chemistry Institute, Ruprecht-Karls University, Heidelberg; Pediatric Clinic, Christian-Albrechts University, Kiel, FRG

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Abstract

Total RNA and mRNA were prepared from cystic fibrosis (CF) and control nasal polyps and nasal epithelial cells. Genomic clones from the chromosomal region of the CF locus were screened by northern blots. A representative cDNA library from nasal polyps was cloned in the vector δgt10. For the construction of a physical genomic map around the CF locus single gene markers were isolated from metaphase 1: 7q2-qter chromosomes by laser micro-dissection and subsequent microcloning. A linkage study with the polymorphic markers met-H, met-D, and pJ3.11 was performed in 53 German CF families with at least 2 children. No significant correlation of any haplotype on the CF chromosomes with the clinical severity of the course of the disease could be observed, which provides evidence that cystic fibrosis is genetically homogeneous.

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The Nasal Polyps as a Tool for Basic Research in Cystic Fibrosis

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