Effect of Trypsin on the Hormonal Regulation of the Fat-Stimulated Human Exocrine Pancreas

O. Olsen Surgical Dept. C, Medical Dept. P, and Dept. of Clinical Chemistry, Rigshospitalet, Copenhagen, Denmark; Dept. of Medical Gastroenterology F, Glostrup Hospital, Copenhagen, Denmark; Dept. of Medical Gastroenterology S, Odense University Hospital, Odense, Denmark; Dept. of Medical and Physiological Chemistry, University of Lund, Lund, Sweden

O. B. Schaffalitzky De Muckadell Surgical Dept. C, Medical Dept. P, and Dept. of Clinical Chemistry, Rigshospitalet, Copenhagen, Denmark; Dept. of Medical Gastroenterology F, Glostrup Hospital, Copenhagen, Denmark; Dept. of Medical Gastroenterology S, Odense University Hospital, Odense, Denmark; Dept. of Medical and Physiological Chemistry, University of Lund, Lund, Sweden

P. Cantor Surgical Dept. C, Medical Dept. P, and Dept. of Clinical Chemistry, Rigshospitalet, Copenhagen, Denmark; Dept. of Medical Gastroenterology F, Glostrup Hospital, Copenhagen, Denmark; Dept. of Medical Gastroenterology S, Odense University Hospital, Odense, Denmark; Dept. of Medical and Physiological Chemistry, University of Lund, Lund, Sweden

C. Erlanson-Albertsson Surgical Dept. C, Medical Dept. P, and Dept. of Clinical Chemistry, Rigshospitalet, Copenhagen, Denmark; Dept. of Medical Gastroenterology F, Glostrup Hospital, Copenhagen, Denmark; Dept. of Medical Gastroenterology S, Odense University Hospital, Odense, Denmark; Dept. of Medical and Physiological Chemistry, University of Lund, Lund, Sweden

C. Palnaes Hansen Surgical Dept. C, Medical Dept. P, and Dept. of Clinical Chemistry, Rigshospitalet, Copenhagen, Denmark; Dept. of Medical Gastroenterology F, Glostrup Hospital, Copenhagen, Denmark; Dept. of Medical Gastroenterology S, Odense University Hospital, Odense, Denmark; Dept. of Medical and Physiological Chemistry, University of Lund, Lund, Sweden

H. Worning Surgical Dept. C, Medical Dept. P, and Dept. of Clinical Chemistry, Rigshospitalet, Copenhagen, Denmark; Dept. of Medical Gastroenterology F, Glostrup Hospital, Copenhagen, Denmark; Dept. of Medical Gastroenterology S, Odense University Hospital, Odense, Denmark; Dept. of Medical and Physiological Chemistry, University of Lund, Lund, Sweden

Abstract

To study the effects of trypsin on the pancreaticobiliary secretion and the release of secretin and cholecystokinin (CCK) to plasma, seven normal subjects were stimulated twice with duodenal perfusates containing 20 mM oleic acid (pH 6.0) with and without 1 g of bovine trypsin added per liter. In addition, six patients with advanced pancreatic insufficiency who received only the oleic acid were compared with eight normal subjects. The concentrations of secretin and CCK in plasma and the pancreatic enzyme and volume secretions were unaffected by the addition of trypsin, but the initial bile acid output and the bicarbonate secretion in the period after gallbladder emptying were reduced during perfusion with trypsin. The severely reduced enzyme secretion in chronic pancreatitis did not influence the basal or oleic acid-stimulated concentrations of the hormones in plasma. The study does not support the hypothesis of a trypsin-mediated negative feedback control of human pancreatic enzyme secretion. Furthermore, the reduced duodenal output of bicarbonate found in response to trypsin is not explained by changes in the release of secretin or CCK.

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Effect of Trypsin on the Hormonal Regulation of the Fat-Stimulated Human Exocrine Pancreas

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Effect of Trypsin on the Hormonal Regulation of the Fat-Stimulated Human Exocrine Pancreas

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