Immunoperoxidase Localization of Apolipoprotein D in Human Enterocytes and Hepatocytes

Abstract

Bouma M-E, de Bandt J-P, Ayrault-Jarrier M, Burdin J, Verthier N, Raisonnier A. Immunoperoxidase localization of apolipoprotein D in human enterocytes and hepatocytes. Scand J Gastroenterol 1988, 23, 477–483

In this study we prepared a pure apolipoprotein D and obtained a specific antiserum to it. The purified apolipoprotein D migrated as a single band of M_r = 29,000 but appeared as five isoforms on isoelectrofocusing. The antiserum did not cross-react with other apolipoproteins. Immunoenzymatic staining revealed the presence of apolipoprotein D in the perinuclear area of the cytoplasm of isolated normal hepatocytes and HepG2 cells. Apolipoprotein D was also localized in intestinal epithelium and in liver cells. The intracellular distribution of apolipoprotein D was similar to that of apolipoprotein B. Our results indicated that apolipoprotein D, like many other circulating apolipoproteins, is synthesized in enterocytes and hepatocytes.

Key Words:

• Apolipoprotein D
• enterocytes
• hepatocytes
• high-density lipoproteins
• immune sera
• immunoenzymology
• immunosorbents