Pharmacokinetics of Rioprostil in Human Plasma as Assayed by Combined Capillary Gas Chromatography-Negative Ion Chemical Ionization Mass Spectrometry

Abstract

Fischer S, Schweer H, Kraft H, Weber P. Pharmacokinetics of rioprostil in human plasma as assayed by combined capillary gas chromatography-negative ion chemical ionization mass spectrometry. Scand J Gastroenterol 1989, 24(suppl 164), 59-62

To investigate the pharmacokinetics of rioprostil in man an assay is developed to analyse levels of rioprostil in plasma. After extraction and purification by solid-phase cartridges rioprostil is measured by negative ion chemical ionization mass spectrometry using a deuterated internal standard. The method is validated by a recovery experiment. Levels of rioprostil in the plasma of four volunteers following a single oral dose of 600 μg rioprostil are found as always below 100 pg/ml. The data presented here suggest that rioprostil is transformed rapidly in man to its more polar metabolites.

Key Words:

• Gas chromatography/mass spectrometry
• human plasma
• rioprostil