Total Biliary Protein, Mucus Glycoproteins, Cyclic-AMP, and Apolipoproteins in the Gallbladder Bile of Patients with Cholesterol Stones and Stone-Free Controls

W. Swobodnik IInd Medical Clinic and Policlinic, Klinikum rechts der Isar, Munich Technical University, Munich; Dept. of Internal Medicine II, Gastroenterology and Nutrition, Ulm, Germany; Dept. of Abdominal Surgery I, University Hospital, Ulm, Germany

H. Wenk IInd Medical Clinic and Policlinic, Klinikum rechts der Isar, Munich Technical University, Munich; Dept. of Internal Medicine II, Gastroenterology and Nutrition, Ulm, Germany; Dept. of Abdominal Surgery I, University Hospital, Ulm, Germany

P. Janowitz IInd Medical Clinic and Policlinic, Klinikum rechts der Isar, Munich Technical University, Munich; Dept. of Internal Medicine II, Gastroenterology and Nutrition, Ulm, Germany; Dept. of Abdominal Surgery I, University Hospital, Ulm, Germany

N. Hagert IInd Medical Clinic and Policlinic, Klinikum rechts der Isar, Munich Technical University, Munich; Dept. of Internal Medicine II, Gastroenterology and Nutrition, Ulm, Germany; Dept. of Abdominal Surgery I, University Hospital, Ulm, Germany

W. Kratzer IInd Medical Clinic and Policlinic, Klinikum rechts der Isar, Munich Technical University, Munich; Dept. of Internal Medicine II, Gastroenterology and Nutrition, Ulm, Germany; Dept. of Abdominal Surgery I, University Hospital, Ulm, Germany

J. Berghold IInd Medical Clinic and Policlinic, Klinikum rechts der Isar, Munich Technical University, Munich; Dept. of Internal Medicine II, Gastroenterology and Nutrition, Ulm, Germany; Dept. of Abdominal Surgery I, University Hospital, Ulm, Germany

Y. Zhang IInd Medical Clinic and Policlinic, Klinikum rechts der Isar, Munich Technical University, Munich; Dept. of Internal Medicine II, Gastroenterology and Nutrition, Ulm, Germany; Dept. of Abdominal Surgery I, University Hospital, Ulm, Germany

R. Bittner IInd Medical Clinic and Policlinic, Klinikum rechts der Isar, Munich Technical University, Munich; Dept. of Internal Medicine II, Gastroenterology and Nutrition, Ulm, Germany; Dept. of Abdominal Surgery I, University Hospital, Ulm, Germany

V. Schusdziarra IInd Medical Clinic and Policlinic, Klinikum rechts der Isar, Munich Technical University, Munich; Dept. of Internal Medicine II, Gastroenterology and Nutrition, Ulm, Germany; Dept. of Abdominal Surgery I, University Hospital, Ulm, Germany

R. Ott IInd Medical Clinic and Policlinic, Klinikum rechts der Isar, Munich Technical University, Munich; Dept. of Internal Medicine II, Gastroenterology and Nutrition, Ulm, Germany; Dept. of Abdominal Surgery I, University Hospital, Ulm, Germany

K. Kuhn IInd Medical Clinic and Policlinic, Klinikum rechts der Isar, Munich Technical University, Munich; Dept. of Internal Medicine II, Gastroenterology and Nutrition, Ulm, Germany; Dept. of Abdominal Surgery I, University Hospital, Ulm, Germany

M. Classen IInd Medical Clinic and Policlinic, Klinikum rechts der Isar, Munich Technical University, Munich; Dept. of Internal Medicine II, Gastroenterology and Nutrition, Ulm, Germany; Dept. of Abdominal Surgery I, University Hospital, Ulm, Germany

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Abstract

The concentrations of total protein, mucus glycoprotein, cyclic-AMP, and apolipoproteins A-I, A-II, and B were determined in the gallbladder bile of patients with cholesterol gallbladder stones and in stone-free controls. The total protein content was significantly increased in gallstone patients (2.03 ± 0.6 versus 1.31 ± 0.67 mg/ ml; p < 0.05), as was the mucus glycoprotein concentration (380 ± 88.5 versus 128 ± 57.2 ug/ml; p < 0.05). The cyclic-AMP concentration in the gallbladder fluid was increased up to 91 ± 20 pmol/100 ul in the gallstone subjects, as compared with 46 ± 26 pmol/100 ul (p < 0.01) in stone-free controls. Cyclic-AMP concentrations correlated positively with the glycoprotein content of the bile in cholesterol gallstone patients (r = 0.66; p <0.05). The apolipoprotein concentrations were determined by the radial immundiffusion technique. The corresponding values for patients with stones and controls were 7.5 ± 0.8 verus 3.0 + 0.8 for Apo A-I (p < 0.025), 10.4 ±0.6 versus 6.3 ± 1.3 for Apo A-II (p < 0.02), and 1.9 ± 0.5 versus 1.6 ± 0.2 mg/dl for Apo B (NS), respectively. Biliary proteins probably play an important role in the nucleation process during the pathogenesis of cholesterol gallbladder stones.

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Total Biliary Protein, Mucus Glycoproteins, Cyclic-AMP, and Apolipoproteins in the Gallbladder Bile of Patients with Cholesterol Stones and Stone-Free Controls

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Total Biliary Protein, Mucus Glycoproteins, Cyclic-AMP, and Apolipoproteins in the Gallbladder Bile of Patients with Cholesterol Stones and Stone-Free Controls

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