12
Views
8
CrossRef citations to date
0
Altmetric
Original Article

Characterization of a Human Trypsin Resistant to Kunitz Soybean Trypsin Inhibitor: Studies of Duodenal Juices after Tube Instillation of Raw Soybean Extract

, , , , &
Pages 589-598 | Received 21 Aug 1990, Accepted 31 Dec 1990, Published online: 08 Jul 2009
 

Abstract

Human duodenal juices collected during tube instillation of raw soybean extract into the duodenum contained free trypsin and free Kunitz soybean trypsin inhibitor (KTI) in the simultaneous presence of trypsin-KTI complexes. It has previously been suggested that this KTI-non-inhibitable trypsin has a general resistance to serine protease inhibitors. Four different trypsin forms have been found and partly characterized by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and isoelectric focusing followed by Western immunoblotting or enzyme staining. In addition, crossed immunoelectrophoresis and affinity chromatography with antibody-coupled gels have been used for identification of free and inhibitor-complexed trypsin.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.