Modification of Reticuloendothelial Function by Muramyl Dipeptide-Encapsulated Liposomes in Jaundiced Rats Treated with Biliary Decompression

Abstract

Rats with 2 weeks of biliary obstruction, with and without 1 week of concomitant biliary decompression relieving the jaundice, were treated with physiologic saline, free muramyl dipeptide (MDP), placebo liposomes, or liposome-encapsulated MDP. Reticuloendothelial system (RES) function was evaluated by blood clearance of intravenously injected ¹²⁵I-labelled Escherichia coli. The corrected phagocytic index (α) after 1 week of biliary decompression returned to normal levels in animals treated with MDP liposomes, whereas RES function was impaired (P < 0.05) in all other jaundiced and biliary-decompressed groups. In the biliary-decompressed, MDP-liposome-treated group, hepatic uptake of radiolabeled bacteria was significantly higher (P < 0.05) and renal entrapment of bacteria was significantly lower (P < 0.05) than in all other jaundiced and biliary-decompressed groups. We conclude that treatment with MDP liposomes improves the otherwise impaired RES function in rats with biliary obstruction and biliary decompression.

Key Words:

• Adjuvant peptide
• choledochoduodenostomy
• cholestasis
• Escherichia coli
• liposome
• obstructive jaundice
• septicemia