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Original Article

Expression of Cytokine mRNA in Gastric Mucosa with Helicobacter pylori Infection

, , , , &
Pages 1153-1159 | Received 20 Dec 1994, Accepted 21 Apr 1995, Published online: 08 Jul 2009
 

Abstract

Background: We have studied the cytokine production patterns in gastric mucosal biopsy specimens with and without the Helicobacter pylori infection, using a reverse transcription-polymerase chain reaction (RT-PCR) method capable of detecting low levels of specific mRNA.

Methods: Total RNA was prepared from biopsy specimens with the acid guanidinium thiocyanate-phenol-chloroform method. cDNA was synthesized by M-MLV RTase and amplified using the oligonucleotide primers specific for interleukin-1β (IL-1β), IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, tumor necrosis factor-α (TNF-α), interferon-α (IFN-α), IFN-β, and IFN-γ by PCR methods.

Results: Although IL-1β and IFN-γ mRNA were detected in most specimens, IL-2, IL-3, IL-4, IL-5, IL-9, TNF-α, and IFN-β mRNA were not detected at all. The expressions of IL-7 and IL-8 mRNA were significantly higher in H. pylori-positive gastritis than in H. pylori-negative normal controls. There was a significant correlation between the expression of IL-8 mRNA and the severity of gastritis both in the antrum and in the corpus. On the other hand, there was a significant correlation between the expression of IL-7 mRNA and the severity of gastritis only in the corpus.

Conclusions: These findings suggest that some cytokines, especially IL-7 and IL-8, play some roles in H. pylori-associated gastritis.

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