6
Views
14
CrossRef citations to date
0
Altmetric
Original Article

Sedimentation of Biliary ‘Sludge’ Effect on Composition of Gallbladder Bile from Patients with Cholesterol, Mixed, or Pigment Stones

, , , , , , , & show all
Pages 273-278 | Received 24 Feb 1995, Accepted 24 Jul 1995, Published online: 08 Jul 2009
 

Abstract

Background: Ultracentrifugation of bile has been used extensively to remove insoluble material such as ‘sludge’ from bile before further studies of cholesterol nucleation. Although it has been recognized that this procedure may affect the composition of gallbladder bile, it has not been studied systematically in different gallstone populations. Therefore, we investigated the concentration of biliary lipids, protein, mucin, and bilirubin before and after ultracentrifugation. Methods: Gallbladder bile samples were aspirated during laparoscopic surgery from 66 patients (35 with cholesterol, 16 with mixed, and 15 with pigment stones). Results: Whereas the concentrations of bile acids, phospholipids, protein, and bilirubin in gallbladder bile did not change significantly after ultracentrifugation, cholesterol (20.6 ± 1.6 to 14.8 ± 1.2 mmol/1) and mucin concentrations (0.99 ± 0.2 to 0.67 ± 0.1 mg/ml) and the cholesterol saturation index (1.68 ± 0.12 to 1.31 ± 0.10) decreased significantly in gallbladder bile from patients with cholesterol stones. Conclusions: Sedimentation of biliary sludge may profoundly affect the composition of gallbladder bile, which has to be considered in studies of cholesterol saturation and nucleation. The cholesterol concentration difference between native and ultracentrifuged biles reflects the insoluble crystalline fraction of cholesterol and may be useful for quantitation of the mass of cholesterol crystals in gallstone-associated bile samples.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.