Bacterial Adherence and Biofilm Formation on Latex and Silicone T-Tubes in Relation to Bacterial Contamination of Bile

A. Koivusalo Fourth Dept. of Surgery and Dept. of Urology, Helsinki University Central Hospital; Päijät-Häme Central Hospital; and Laboratory of Enteric Pathogens and Laboratory of Hospital Infections, The National Public Health Institute, Helsinki, Finland

H. Mäkisalo Fourth Dept. of Surgery and Dept. of Urology, Helsinki University Central Hospital; Päijät-Häme Central Hospital; and Laboratory of Enteric Pathogens and Laboratory of Hospital Infections, The National Public Health Institute, Helsinki, Finland

M. Talja Fourth Dept. of Surgery and Dept. of Urology, Helsinki University Central Hospital; Päijät-Häme Central Hospital; and Laboratory of Enteric Pathogens and Laboratory of Hospital Infections, The National Public Health Institute, Helsinki, Finland

A. Siitonen Fourth Dept. of Surgery and Dept. of Urology, Helsinki University Central Hospital; Päijät-Häme Central Hospital; and Laboratory of Enteric Pathogens and Laboratory of Hospital Infections, The National Public Health Institute, Helsinki, Finland

J. Vuopio-Varkila Fourth Dept. of Surgery and Dept. of Urology, Helsinki University Central Hospital; Päijät-Häme Central Hospital; and Laboratory of Enteric Pathogens and Laboratory of Hospital Infections, The National Public Health Institute, Helsinki, Finland

M. Ruutu Fourth Dept. of Surgery and Dept. of Urology, Helsinki University Central Hospital; Päijät-Häme Central Hospital; and Laboratory of Enteric Pathogens and Laboratory of Hospital Infections, The National Public Health Institute, Helsinki, Finland

K. Höckerstedt Fourth Dept. of Surgery and Dept. of Urology, Helsinki University Central Hospital; Päijät-Häme Central Hospital; and Laboratory of Enteric Pathogens and Laboratory of Hospital Infections, The National Public Health Institute, Helsinki, Finland

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Abstract

Background: T-tube-related bacteriobilia causes infectious complications and obstruction. To prevent these, the choice of T-tube material may be of importance. Methods: Transected common bile ducts (CBDs) of 17 piglets were sutured over latex or silicone T-tubes, or without a T-tube. Results: After 6 weeks bacteriobilia was found in all of 12 CBDs with and in 1 of 5 CBDs without a T-tube (p < 0.05). By scanning electron microscopy (SEM) four of five latex and none of five silicone T-tubes had bacterial biofilms (p < 0.05). All tubes remained patent. Segments of T-tubes were incubated with five different bacterial strains. Sonication and SEM showed that 0.1–1.1% of 10⁷ colony-forming units of inoculum adhered to T-tubes. Two to six times more bacteria adhered to latex than to silicone (p < 0.05). Conclusions: Silicone offers better long-term patency than latex. Less infectious complications occur if T-tubes are omitted.

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Bacterial Adherence and Biofilm Formation on Latex and Silicone T-Tubes in Relation to Bacterial Contamination of Bile

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Bacterial Adherence and Biofilm Formation on Latex and Silicone T-Tubes in Relation to Bacterial Contamination of Bile

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