Flagellin Gene Diversity among Helicobacter pylori Strains and IL-8 Secretion from Gastric Epithelial Cells

U. Ohta-Tada Dept. of Internal Medicine 6 and Dept. of Infectious Diseases, School of Medicine, Tokai University, Bohseidai, Isehara, Tokyo, Japan; Dept. of Bacteriology, Kyorin University School of Medicine, Mitaka, Tokyo, Japan

A. Takagi Dept. of Internal Medicine 6 and Dept. of Infectious Diseases, School of Medicine, Tokai University, Bohseidai, Isehara, Tokyo, Japan; Dept. of Bacteriology, Kyorin University School of Medicine, Mitaka, Tokyo, Japan

Y. Koga Dept. of Internal Medicine 6 and Dept. of Infectious Diseases, School of Medicine, Tokai University, Bohseidai, Isehara, Tokyo, Japan; Dept. of Bacteriology, Kyorin University School of Medicine, Mitaka, Tokyo, Japan

S. Kamiya Dept. of Internal Medicine 6 and Dept. of Infectious Diseases, School of Medicine, Tokai University, Bohseidai, Isehara, Tokyo, Japan; Dept. of Bacteriology, Kyorin University School of Medicine, Mitaka, Tokyo, Japan

T. Miwa Dept. of Internal Medicine 6 and Dept. of Infectious Diseases, School of Medicine, Tokai University, Bohseidai, Isehara, Tokyo, Japan; Dept. of Bacteriology, Kyorin University School of Medicine, Mitaka, Tokyo, Japan

Abstract

Background: To clarify the pathological functions of the virulence factors of Helicobacter pylori, a comparative analysis was carried out on the relationship between motility, flagellar gene polymorphism, vacuolating cytotoxin (VT) production and interleukin-8 (IL-8) induction. Methods: Twenty-five strains were examined for restriction fragment length polymorphism (RFLP) of the flagellin gene. Motility was measured using semisolid agar plates. Cytotoxicity was assayed using RK-13 cells. IL-8 secretion was assessed by the enzyme-linked immunosorbent assay (ELISA) methods. Results: H. pylori was classified into four groups according to their flagellar RFLP. No differences were noted in motility or VT production among the four groups, but a significant difference was noted in IL-8 induction. In addition, highly motile strains produced more IL-8. Conclusion: This flagellar genetic polymorphism may be associated with IL-8 induction.

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Flagellin Gene Diversity among Helicobacter pylori Strains and IL-8 Secretion from Gastric Epithelial Cells

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Flagellin Gene Diversity among Helicobacter pylori Strains and IL-8 Secretion from Gastric Epithelial Cells

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