Molecular characterization of oxacillinases and genotyping of invasive Acinetobacter baumannii isolates using repetitive extragenic palindromic sequence-based polymerase chain reaction in Ankara between 2004 and 2010

Abstract

Background: Multidrug-resistant Acinetobacter baumannii (MDRAB) is an increasing problem worldwide. We aimed to determine the antibiotic susceptibility, diversity of oxacillinases, and molecular types of MDRAB. Methods: A total of 100 non-duplicate A. baumannii blood culture isolates were evaluated. Antimicrobial susceptibilities of the isolates were determined according to the standard Clinical and Laboratory Standards Institute (CLSI) broth microdilution method. Colistin, doripenem, and tigecycline susceptibilities were analyzed by E-test. The presence of bla_OXA-23-like, bla_OXA-24-like, bla_OXA-51-like, and bla_OXA-58-like genes was investigated by multiplex polymerase chain reaction (PCR). Typing of A. baumannii isolates was performed using repetitive extragenic palindromic sequence-based PCR (rep-PCR; DiversiLab). Results: Most isolates were susceptible to colistin (98% susceptible) and tigecycline (94% susceptible), whereas fewer isolates were susceptible to imipenem, meropenem, and doripenem (17%, 17%, and 18% susceptible, respectively). Carbapenem resistance was associated with the presence of bla_OXA-23-like (31% of isolates) and bla_OXA-58-like (23% of isolates) genes. The occurrence of isolates carrying bla_OXA-58-like genes increased between y 2004 and 2009, but decreased in 2010. In contrast, isolates with bla_OXA-23-like genes increased during the 2008–2010 period. Out of 100 isolates, 62 were categorized into 13 major rep-PCR patterns, with the highest prevalence in pattern 1 (10 isolates), followed by patterns 2 and 3 (9 isolates each). Conclusions: Carbapenem-resistant invasive A. baumannii isolates carrying the bla_OXA-23-like gene became more prevalent and replaced isolates carrying the bla_OXA-58-like carbapenemase gene through the 7 y. Rep-PCR genotyping of these strains confirmed that ongoing MDRAB resulted from a long-term persistence and mixture of several clusters.

Keywords::

• Acinetobacter baumannii
• OXA
• rep-PCR

Acknowledgement

We would like to thank Dr Ahmet Süel (bioMérieux) for technical help.

Declaration of interest: This study was supported by the Hacettepe University Scientific Research Unit with 2 supportive grant projects. (Project no. 09D05406001(2009) and Project no. 011D04406002 (2011)).

No commercial relationship or potential conflict of interest exists related to the submission.