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Original Article

A New Peptide for Human Parvovirus B19 Antibody Detection

, &
Pages 597-603 | Published online: 08 Jul 2009
 

Abstract

A serological assay for human parvovirus B19 was developed. Linear overlapping synthetic peptides were synthesised according to parts of open reading frames 1 and 2. A region at the N-terminus of viral protein VP2 detected serological reactivity in indirect enzyme-linked immunosorbent assays for IgG and IgM with known seropositive human sera. A cyclized peptide taken from this region, amino acids 284–307, gave the best selective reactivity with seropositive and seronegative sera. The peptide assay appears suitable for further studies of B19 infections and their complications.

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