Function of Canine Renal Autografts After Extracorporeal Preservation

Abstract

The function of extracorporeally preserved and replanted dog kidneys has been studied by determinations of serum creatinine, plasma clearances of PAH and inulin and blood flow using the xenon-133 wash-out technique. The kidneys were also examined histologically. After excision of the kidneys a cool and bloodless state was immediately induced by perfusion with 5% Rheoma-crodex solution followed by a buffered sugar solution both at +5°C temperature. After preservation periods between 5 and 96 h at this temperature replantation to the same animal was done. Seven animals were followed for 7 weeks and another group of 7 animals for 1 year. The results of the functional studies show that preservation for up until 20 h with this simple method is compatible with reasonable function of the solitary transplanted kidney. During the 1 year follow up most of the kidneys increased their function. The histologic examinations disclosed degenerative tubular changes, which healed during the 1 year follow up. Moderate signs of glomerular damage were noted which were not reversible during the 1-year follow-up.