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Original Article

Kidney Preservation: II. Liberation of Enzymes and Urea from Preserved Kidneys

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Pages 280-283 | Accepted 05 Oct 1971, Published online: 09 Jul 2009
 

Abstract

A sensitive (further developed) method is described of determining kidney damage caused by ischemia. By using 1/2 ml perfusion fluid per g kidney weight and a perfusion height of 100 cm, it is possible to obtain a sensitive parameter by the determination of LDH in the perfusate. This parameter is a sensitive indicator of whether or not the kidney has had a long (especially warm) ischemia time. While pre-treatment of a ludney donor with chlorpromazine is of importance for the subsequent renal function after transplantation, addition of chlorpromazine to the perfusion fluid seems to be without significance. Urea was also found in perfusates from preserved kidmys. Determination of this parameter has some advantages compared with determination of enzyme loss.

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