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Original Article

Metabolism of Estrone Sulphate by Benign Prostatic Hyperplasia in Vitro

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Pages 135-137 | Received 06 Apr 1979, Published online: 15 Feb 2010
 

Abstract

Homogenates of benign prostatic hyperplastic (BPH) tissue and abdominal muscle were incubated with [3H] estrone sulphate in the presence of NADH and NADPH. The metabolites were extracted with chloroform, isolated by thin layer chromatography (TLC) and identified by addition of 14C-labelled standards followed by sequential chromatography and acetylation. Both tissues transformed the substrate into [3H] estrone and [3H] estradiol-17β, but BPH tissue by far exceeded the abdominal muscle in this respect. Estriol and other polar metabolites could not be detected. The specific ability of BPH tissue to convert the major peripheral estrogen estrone sulphate into the terminal biologically active estrogen estradiol-17β further indicates an etiological role of estrogens in the development of human BPH.

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