24
Views
39
CrossRef citations to date
0
Altmetric
Original Article

Stimulation of Sperm Progressive Motility by Organelles in Human Seminal Plasma

&
Pages 85-90 | Received 15 Apr 1981, Published online: 15 Feb 2010
 

Abstract

A simple method for objective determination of sperm progressive motility is described. Comparisons were made between results with this method and subjective grading of motility. Although congruence between the two methods was demonstrated, the subjective evaluation had obvious disadvantages. In suspensions of NaCl-washed spermatozoa, seminal plasma was required for progressive motility. Various isotonic salt solutions without seminal plasma were ineffective in this respect. Washed spermatozoa, however, were always motile in a medium consisting of pellet II, obtained from ultracentrifugation of human seminal plasma, thoroughly mixed with isotonic NaCl solution. The progressive sperm motility was two to three times as great in that medium as in seminal plasma mainly devoid of organelles. Magnesium, calcium and zinc ions, in the presence of seminal plasma, could exert stimulatory and inhibitory effects on sperm progressive motility, depending on the concentration of each divalent cation. Magnesium gave the greatest stimulatory and zinc the greatest inhibitory effect.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.