Uremic Acidosis and Intracellular Buffering

Abstract

Skeletal muscle biopsies were performed in 16 controls and 15 non-dialysed end-stage chronic renal failure (CRF) patients presenting untreated metabolic acidosis. Intracellular bicarbonate, pH. water compartments and electrolytes were determined. In 8 of 15 patients muscle ATP and lactate were measured. Intracellular bicarbonate (HCO_3i) and pH (pH_i) were obtained by means of muscle total carbon dioxide method: a significant (p < 0.001) reduction in both intracellular acid-base indexes was found in all patients (pH, 6.82±13 vs. 7.04±0.05; HCO_3i 6.28±2.07 vs. 11.86±0.87). Total muscle as well as extracellular water was increased. Muscle sodium and chloride contents were also increased, while no change in potassium and magnesium was detected. A significant decrease of both muscle ATP and lactate was found. The data lead to the conclusion that chronic retention of acids in CRF results in-a depletion of the muscle buffer pool and consequently in intracellular acidosis: the latter could be the main cause of the cell energy metabolism derangement described in uremia.

Key Words:

• uremia
• acidosis
• intracellular pH
• intracellular buffers
• energy metabolism