Influence of Escherichia Coli on Urease-Induced Crystallisation in Human Urine

Abstract

Urease was added to urines inoculated with Escherichia coli 24 hours earlier and to control urines not inoculated with E. coli. The inoculation did not change the concentration of the measured urine components. The urease-induced ammonium ion production and pH increase was reduced in E. coli-inoculated urines compared to control urines. This suggests that E. coli can inhibit urease. The precipitation of both phosphate and magnesium on glass rods inserted in the urine was reduced with 40–50% in the E. coli-inoculated urines. The results demonstrate that E. coli can influence urease-induced crystallisation.

Key words:

• Escherichia coli
• urease-induced crystallisation
• human urine
• infection stones