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Original Article

Evaluation of Non-invasive Clinical Samples in Chronic Chlamydial Prostatitis by using In situ Hybridization

, , , , , & show all
Pages 449-451 | Received 29 Jun 1996, Accepted 02 Jan 1997, Published online: 09 Jul 2009
 

Abstract

Seventy-eight non-invasive prostate specimens collected from patients with chronic non-bacterial prostatitis were evaluated by in situ hybridization (IH) for evidence of Chlamydia trachomatis. Intracellular Chlamydia bodies were detected in 18 of 78 cases (20.6%). Homogeneous blue-black bodies in the cellular cytoplasm were accepted as in situ positive. Chlamydia1 antigen detected by enzyme immuno assay (EIA) was positive in 12 cases (13.7%), but only nine of them were positive by IH. Our study confirms previous reports implicating C. trachomatis as an aetiological agent in chronic non-bacterial prostatitis, and underscores the applicability of DNA probes for detection and identification of C. trachomaris in prostatic materials.

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