Survival After High-Dose Intraperitoneal Infusion of Glycine Solution in the Mouse

Abstract

We used an animal model to study whether glycine prompts death after absorption of irrigating fluid by the extravascular route. Mice were divided into groups of 10 and given an intraperitoneal infusion of 200, 225, 250, 275 or 300 ml/kg of glycine 1.1%, 1.5% or 2.2%. With all infused volumes, the chance of survival was better after infusion of glycine 1.1% and glycine 1.5% than after glycine 2.2%. The overall death rates were 10%, 16% and 46%, respectively. Logistic regression analysis showed that the outcome was significantly and independently affected by both the glycine concentration and the infused volume. We conclude that the glycine concentration of an irrigating fluid deposited in the peritoneal cavity is important to the chance of survival. The poorest chance is obtained with glycine 2.2%.

Key Words:

• irrigation
• adverse effects
• glycine
• toxicity
• osmolar concentration