Binding of Nitrogen Containing Compounds to Microsomal Cytochromes

Abstract

1. The occurrence of cytochrome P-450 in biological tissues and its probable function in mixed-function-oxidase type reactions is briefly discussed.

2. The binding of lipophilic ligands, such as ethylisocyanide, to the reduced form of cytochrome P-450 is mentioned.

3. Factors affecting the type I and type II binding spectra of nitrogen-containing compounds to the oxidized form of cytochrome P-450 are reviewed.

4. The precise nature of the two different types of binding to the haeme centre of the cytochrome is discussed.