Publication Cover
Xenobiotica
the fate of foreign compounds in biological systems
Volume 1, 1971 - Issue 2
5
Views
3
CrossRef citations to date
0
Altmetric
Research Article

Effect of (—)-Emetine on Liver Microsomal Protein Synthesis in Partially Hepatectomized Rats

, &
Pages 131-141 | Received 07 Jun 1971, Published online: 15 Sep 2008
 

Abstract

1. In the early sequential response to partial hepatectomy there is an initial inhibition of liver microsomal protein-synthesizing activity in vitro comparable in duration and degree to that occurring after administration of (—)-emetine (18 μmol/kg) to intact female rats. This initial inhibition is followed by a prolonged phase of stimulated amino acid incorporating activity by liver microsomal fractions prepared from donor animals at later times.

2. There is a delayed appearance of the stimulatory phase of microsomal amino acid incorporating activity in vitro when donor animals are treated with (—)-emetine immediately after partial hepatectomy. This may be related to the finding that in these animals hepatic enzymes involved in the biosynthesis of DNA, such as deoxycytidylate deaminase and thymidine kinase, are not stimulated in the expected way.

3. By separating the two challenges and superimposing 24 h treatment with (—)-emetine on 48 h regeneration, or 24 h regeneration on 48 h (—)-emetine treatment, the liver microsomal protein-synthesizing activity can be stimulated to a greater extent than with either challenge alone. This greater stimulation occurs after the second challenge has also exerted its initial inhibitory phase response, which implies that an initial inhibitory phase may be involved in the mechanism for initiating the regenerative response.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.