Electron Transport Systems of Lung Microsomes and Their Physiological Functions Enzymic Hydroxylation of Aniline and Steroids

Abstract

1. The hydroxylation of aniline by rabbit lung microsomes to p-aminophenol required oxygen and NADPH, and was inhibited by menadione, ferricytochrome c and carbon monoxide.

2. NADH was a less effective electron donor than NADPH in this reaction, but its addition significantly increased the yield of p-aminophenol formed in the presence of NADPH.

3. 4,16-Androstadien-3-one and 3β-hydroxy-5-androsten-17-one were also metabolized by lung microsomes to the same products as are formed by hepatic microsomes.