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Xenobiotica
the fate of foreign compounds in biological systems
Volume 5, 1975 - Issue 7
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Research Article

An Incubation Flask to Study Metabolism of Foreign Compounds by Intestinal Microflora

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Pages 407-411 | Received 04 Jan 1975, Published online: 22 Sep 2008
 

Abstract

1. The design and procedure for use of an all-glass incubation flask which facilitates the production and maintenance of an anaerobic environment suitable for the study of drug metabolism by intestinal microflora is described.

2. Evaluation was based on monitoring available oxygen with an oxygen electrode.

3. Removal of air by the combination of replacement with hydrogen, a room temperature catalyst and a flushing technique, produced low oxygen levels comparable to vacuum systems without the need for adverse pressure changes.

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