Aniline Metabolism in Two Species of Arachnida: Water-Soluble Metabolites

Abstract

1. The metabolism of [¹⁴C]aniline in the cattle tick, Boophilus microplus (Canestrini) (at 40 and 300 μg per animal) and in the spider, Nephila plumipes (at 40 μg per 100 mg) were studied.

2. In both species hydroxylation occurred yielding both free and conjugated o- and p-aminophenols.

3. The water-soluble metabolites affording aniline on acid hydrolysis were examined, and γ-glutamylanilide (23% of dose) was identified as a tick metabolite (40 μg dose). This metabolite was not found in extracts of dosed spiders.