19-Nortestosterone, a Model for the Use of Anabolic Steroid Conjugates in Raising Antibodies for Radioimmunoassay

Abstract

1. The raising of antibodies in Soay sheep to an immunogenic conjugate of 19-nortestosterone-3-(O-carboxymethyl)oxime with bovine serum albumin is described.

2. High titre plasma so obtained can be used for the radioimmunoassay of 19-nortestosterone.

3. Cross-reactivities of the antibody with endogenous steroids is confined to testosterone (20-25%) and 17β-estradiol (1-2%). Cross-reactivities with a range of other 17β-hydroxy synthetic anabolic steroids and with some potential 17β-hydroxy metabolites of 19-nortestosterone vary between 2-3% for 5β-estrane-3α,17β-diol and 26% for 1-dehydrotestosterone. There is limited cross-reactivity (ca. 1-2%) with anabolic steroids with 17β-hydroxy, 17α-methyl substituents.

4. 19-Nortestosterone (phenyl propionate) administered to a horse (1 mg/kg, intra-muscular) gives urinary metabolites readily detectable by radioimmunoassay, for more than 4 days.