The Formation of Phenylethane-1,2-diol 2-Acetate in the Metabolism of Styrene Oxide by Rabbit Liver Microsomes in vitro

Abstract

1. Phenylethane-1,2-diol 2-acetate has been isolated from the incubation of styrene oxide or styrene diol with liver microsomes of phenobarbital-pretreated rabbits.

2. Observed ratios of phenylethane-1,2-diol to its 2-acetate in the incubation mixtures ranged between 19:1 and 5 : 1 with different microsomal preparations and different periods of incubation.

3. The formation of phenylethane-1,2-diol 2-acetate may be a source of errors in the radiometric determination of epoxide hydrase activity in microsomes.