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Xenobiotica
the fate of foreign compounds in biological systems
Volume 9, 1979 - Issue 9
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Original Article

The Formation of Cytochrome P-450-Metabolic Intermediate Complexes from Amines, in the Isolated Perfused Rat Liver

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Pages 547-554 | Received 02 Feb 1979, Published online: 30 Sep 2009
 

Abstract

1. Cytochrome P-450-metabolic intermediate (MI) complexes were formed from SKF 525-A, propoxyphene, acetylmethadol, noracetylmethadol, norbenzphetamine, and N-hydroxyamphetamine, but not methadone, in the isolated perfused rat liver.

2. The amount of MI complex from SKF 525-A (8% of the cytochrome P-450) after 1 h exceeded that for all other compounds (1-2%).

3. Both MI complex and residual substrate contributed to the inhibition of mixed-function oxidase activity observed. No substrate altered the total cytochrome P-450 concentration or NADPH-cytochrome c reductase activity.

4. The formation of MI complexes in isolated perfused liver corresponds to that seen m whole animals, and contrasts with that seen in microsomal preparations.

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