Publication Cover
Xenobiotica
the fate of foreign compounds in biological systems
Volume 11, 1981 - Issue 7
83
Views
62
CrossRef citations to date
0
Altmetric
Research Article

Differences in the biotransformation of a 17β-hydroxylated steroid, trenbolone acetate, in rat and cow

, , &
Pages 489-500 | Received 01 Dec 1980, Published online: 22 Sep 2008
 

Abstract

1. The metabolism of trenbolone acetate, 17β-acetoxyestra-4,9,11-trien-3-one (TBA), an anabolic compound used as a growth promoter, was compared in rat and cow.

2. [6,7-3H] TBA was injected i.v. into rats and a heifer, and bile was collected for 24 h. In both species, the bile was the major route of excretion. TBA undergoes an extensive hydrolysis to 17β-hydroxyestra-4,9,11-trien-3-one and the unchanged compound was not detected, but subsequent major metabolic pathways are different in the two species.

3. In the rat, oxidation of the 17β-hydroxyl to the 17-oxo group and hydroxylation in the 16α-position are the major routes. The three major metabolites are 17β-hydroxyestra-4,9,11-trien-3-one, 16α,17β-dihydroxyestra-4,9,11-trien-3-one and 16α-hydroxyestra-4,9,11-trien-3,17-dione.

4. In the heifer, 17α-epimerization is the major pathway and the main metabolite is the 17α-hydroxyestra-4,9,11-trien-3-one.

5. In both species, estra-4,9,11-trien-3,17-dione and the other metabolites, resulting either from hydroxylation in 1, 2, 6β, 16α or 16β positions, or from aromatization of the A ring, were minor products.

6. Overall, 60% of the 3-oxotriene structures identified in the rat bile were 17β-hydroxylated and the remainder were 17-keto metabolites, whereas in the heifer bile 90% were 17α-hydroxylated compounds.

7. Thus, in bovine species, the major pathway is similar to those of testosterone or 17β-estradiol which are mainly excreted as their 17α-epimers. This epimerization strongly decreases the biological potency, as with the natural 17β-hormones, and leads to detoxication of tissue residues.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.