Bacterial degradation of aminopyrine

Abstract

1. Four strains of bacteria growing with aminopyrine as sole source of carbon were isolated from soil and were identified as strains of Phenylobacterium immobilis.

2. Strain M₁₃ and strain E, the type species of Phenylobacterium immobilis (DSM 1986), which had been isolated by enrichment with chloridazon (5-amino-4-chloro-2-phenyl-2H-pyridazin-3-one) were used to investigate the bacterial degradation of aminopyrine.

3. Three metabolites were isolated and identified as: 4-

4. An enzyme extract from cells of strain M₁₃ was shown to further metabolize the catechol derivative of aminopyrine, with the formation of 2-pyrone-6-carboxylic acid.

5. Results indicate that the benzene ring of aminopyrine is the principal site of microbial metabolism.