The influence of L-4-oxalysine on carbon tetrachloride-induced changes in drug-metabolizing enzyme activity of mouse liver

Abstract

1. Carbon tetrachloride (0.2 ml/kg) and L-4-oxalysine (200 mg/kg per day for two days) were administered to male mice separately, and together, and the liver drug-metabolizing parameters measured.

2. Carbon tetrachloride alone depleted hepatic microsomal protein content by 50% and cytosolic sulphydryl compounds by 90%; oxalysine alone had no effect.

3. Microsomal cytochrome P-450 was decreased by 60%, cytochrome b₅ by 30%, ethylmorphine N-demethylation by 50% and 7-ethyoxycoumarin O-deethylation by 80% following carbon tetrachloride administration; oxalysine had no effect on these losses.

4. After administration of carbon tetrachloride, cytosolic glutathione S-transferase activity was decreased by 16%; this effect was not seen when oxalysine and carbon tetrachloride were given together. Oxalysine alone slightly increased this enzyme activity.