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Abstract
1. An investigation was carried out on the hydrolytic activity of the intestinal mucus/flora on phenylglucuronide in the bile of goldfish.
2. Approximately 79% of the total phenylglucuronide (3.4 μmol) in the bile was hydrolysed after 16 h incubation with the intestinal mucus/flora.
3. Of the total phenol in the aquarium water of goldfish exposed previously to phenol for 48 h, 41% was found to be phenyglucuronide when fish were placed in a phenol-free medium and were dosed hourly for 8 h with D-saccharic acid 1,4-lactone to inhibit β-glucuronidase activity in the intestine.