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Xenobiotica
the fate of foreign compounds in biological systems
Volume 14, 1984 - Issue 7
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Original Article

The measurement of FAD-containing mono-oxygenase activity in microsomes containing cytochrome P-450

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Pages 515-520 | Received 28 Nov 1983, Published online: 30 Sep 2009
 

Abstract

1. Antibodies to NADPH-cytochrome P-450 reductase have been used to essentially abolish the contribution of cytochrome P-450 to xenobiotic metabolism by mammalian microsomes. This permits the determination of the activity of the FAD-containing monooxygenase and the stoichiometry between substrate, O2 and NADPH, in the microsomal membrane, and in the absence of cytochrome P-450-dependent activity.

2. FAD-containing mono-oxygenase oxidation rates were determined for sulphur- and nitrogen-containing substrates, including: thiols; sulphides; thioamides; primary, secondary and tertiary amines; hydrazines.

3. Although the enzyme in mouse, rabbit, rat and pig microsomes displays similar substrate specificity, some catalytic characteristics are different between species and tissues.

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