Assay of pyrethroid-hydrolysing esterases using (1,R)-cis-3-(2,2-dibromovinyl)-2,2-dimethyl cyclopropane carboxylates as substrates

Abstract

1. p-Nitrophenyl-, α-naphthyl-, and β-naphthyl-(1,R)-cis-3-(2,2-dibromovinyl)2,2-dimethylcyclopropane carboxylates are proposed as pyrethroid-like substrates for the detection of pyrethroid esterases (PyEs).

2. The substrates have been used in a spectrophotometric assay of PyEs and in a staining method to locate PyEs on polyacrylamide electrophoresis.