Lipoprotein-mediated transfer of 2,4,5,2′,4′,5′-hexachlorobiphenyl into cultured human cells

Abstract

1. The very low density, low density and high density lipoproteins (VLDL, LDL, HDL), centrifugally separated from human plasma treated with 2,4,5,2′,4′,5′-hexachloro[¹⁴C]biphenyl (¹⁴C-HCB) contained approximately 50% of the ¹⁴C-HCB.

2. Normal skin fibroblasts were incubated at 4°C or 37°C for varying times with medium containing 10% serum, LDL or HDL labelled with ¹⁴C-HCB. Cellular incorporation of ¹⁴C-HCB from serum was temperature-dependent and occurred mainly during the first 30 minutes. Cellular accumulation of ¹⁴C-HCB from isolated lipoproteins was also rapid and was more efficient from HDL than from LDL or serum. Accumulation from HDL was concentration-dependent and temperature-dependent.

3. The efflux of ¹⁴C-HCB from cells into serum- or lipoprotein-containing medium occurred very rapidly and was most effective in the presence of 20% serum. The order of efficiency in removal of HCB from cells was 20% serum, 50 μg LDL protein/ml, and 120 μg HDL protein/ml. Little or no efflux from cells occurred into serum-free, lipoprotein-free medium.

4. HDL may be involved in the delivery of HCB to cells, a role in contrast to the generally accepted theory that HDL transports lipids from cells.