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Xenobiotica
the fate of foreign compounds in biological systems
Volume 18, 1988 - Issue 6
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Original Article

Metabolizing systems in cell culture cytotoxicity tests

, &
Pages 649-656 | Published online: 30 Sep 2009
 

Abstract

1. The addition of 9000 g supernatant of rat liver homogenate (S9) or rat liver microsomal fractions to a cytotoxicity test system using BCL-D1 cells has been investigated.

2. The choice of culture medium influenced the intrinsic cytotoxicity of the metabolising system to the BCL-D1 cells. Use of Ham's F10 nutrient mixture resulted in greater cytotoxicity compared with several other media.

3. Microsomal fractions provided greater cytochrome P-450 dependent activation of cyclophosphamide and were less cytotoxic than S9.

4. Direct-acting toxic compounds, such as p-aminophenol, were less toxic in the presence of a metabolising system. This was due to protein-binding rather than enzymic detoxification.

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