Publication Cover
Xenobiotica
the fate of foreign compounds in biological systems
Volume 19, 1989 - Issue 9
77
Views
39
CrossRef citations to date
0
Altmetric
Research Article

Pharmacokinetics and biotransformation of diethylene glycol and ethylene glycol in the rat

, &
Pages 961-979 | Received 03 Sep 1988, Accepted 05 May 1989, Published online: 22 Sep 2008
 

Abstract

1. 14C-Diethylene glycol (DEG), administered orally to rats at 1, 5, and 10 ml/kg, gave elimination half-lives of 6, 6, and 10 h, respectively, from urinary excretion data. Half-logarithmic plots of urinary 14C excretion rates versus time indicated zero-order elimination for the first 9 and 18 h after oral doses of 5 and 10 ml of 14C-DEG/kg, respectively. 14C-DEG urinary elimination kinetics changed into first-order 6, 9, and 18 h after oral doses of 1, 5, and 10 ml/kg, with a half-life of 3h.

2. After oral doses of 3 and 5 ml ethylene glycol (EG)/kg, half-lives of 4.5 and 4.1 h were estimated from cumulative urinary excretion data for non-metabolized EG. A half-life of 2h was determined from half-logarithmic plots of urinary excretion rates of non-metabolized EG after the same oral doses of EG.

3. The urinary concentrations of non-metabolized DEG and its metabolite, 2-hydroxyethoxyacetic acid (2-HEAA), determined by high-resolution n.m.r. spectroscopy in the urine of rats doses with DEG were 61–68% and 16–31% dose, respectively.

4. Urinary concentrations of non-metabolized EG and its metabolite, glycolic acid (GA), determined by n.m.r., gave 62–67% for non-metabolized EG and 28.7% for GA following oral doses of EG.

5. Oxidation of DEG and EG in rats was accompanied by a change of urinary pH, reflecting metabolic acidosis.

6. Comparison of the Km for DEG oxidation in vitro by ADH with that of ethanol oxidation, showed a 680-fold difference in substrate affinity. DEG inhibited ethanol oxidation non-competitively, the Ki being 0.44 m.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.