Abstract
1. Three dogs were treated i.v. with cannabidiol (CBD) and urine collected at intervals to 30h.
2. Metabolites were extracted, converted into trimethylsilyl (TMS) derivatives and examined by g.l.c.-mass spectrometry.
3. The major metabolites excreted at early times were identified as the phenol glucosides of 4″-hydroxy-CBD, 5″-hydroxy-CBD and 6-oxo-CBD.
4. These three oxidized metabolites were not found unconjugated, and none of the free oxidized metabolites in urine were found conjugated with glucose.
5. The conjugates were hydrolysed by β-glucuronidase Type HP-2 from Helix pomatia and acid phosphatase but not by β-glucuronidase Type VII from E. coli. Differential reactivity towards α- and β-glucosidase indicated that they possessed the β-configuration.