Metabolism of illudin S, a toxic substance of Lampteromyces japonicus. IV. Urinary excretion of an illudin S metabolite in rat

Abstract

The urinary excretion of a metabolite of illudin S after oral administration to rat has been studied.

2. From an ethyl acetate extract of urine, metabolite 1, a cyclopropane ring-cleavage compound, was identified by liquid chromatographic-mass spectrometric analysis

3. A hplc method for determination of the metabolite in rat urine was developed with extraction using Sep-Pak C_18M cartridge followed by liquid-liquid extraction.

4. The metabolite excreted during 3 days after administration of illudin S to rat amounted to approximately 10–19% of the dose as free form and 3–5% of the dose as glucuronide.