Induction of zebrafish (Brachydanio rerio) P450 in vivo and in cell culture

P. Collodi Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR, USA

C. L. Miranda Department of Agricultural, Chemistry Oregon State University, Corvallis, OR, USA; Marine/Freshwater Biomedical Sciences Center, Oregon State University, Corvallis, OR, USA

X. Zhao Department of Agricultural, Chemistry Oregon State University, Corvallis, OR, USA

D. R. Buhler Department of Agricultural, Chemistry Oregon State University, Corvallis, OR, USA; Marine/Freshwater Biomedical Sciences Center, Oregon State University, Corvallis, OR, USA

D. W. Barnes Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR, USA

Abstract

1. Induction of zebrafish P450 by 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD) was studied in liver tissue, primary liver cell culture and multipassage cell culture derived from zebrafish haploid and diploid embryos and liver.

2. TCDD induced two hepatic proteins (54 and 50 kDa) in vivo which were recognized by anti-trout P4501A1 IgG. The 54-kDa protein was induced by TCDD in primary and multipassage hepatocyte cultures and in haploid and diploid embryo-derived cells. The proteins in liver homogenates were not induced by aqueous exposure of zebrafish to β-naphthoflavone (BNF).

3. Homogenates of zebrafish liver, cultured hepatocytes and embryo-derived cells also exhibited increased ethoxyresorufin O-deethylase (EROD) and 7-12-dimethyl-benz[a]anthracene (DMBA) hydroxylase activity following TCDD exposure.

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Induction of zebrafish (Brachydanio rerio) P450 in vivo and in cell culture

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Induction of zebrafish (Brachydanio rerio) P450 in vivo and in cell culture

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