Metabolism of illudin S, a toxic substance of Lampteromyces japonicus: urinary excretion of mercapturic acids in rat

Abstract

1. The urinary excretion of the mercapturic acids of illudin S after oral administration to rat has been studied.

2. From Ic-ms/ms analysis of methanolic extracts of lyophilized rat urine, stereoisomeric mercapturic acids were detected.

3. The mercapturic acids excreted 3 days following administration amounted to approximately 0.39–0.73% of the administered dose.

4. In vitro glutathione conjugation of illudin S by subcellular fractions was also examined.

5. No significant increases in the formation of glutathione adducts were observed in any subcellular fractions examined.